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pkn2 mab  (R&D Systems)


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    R&D Systems pkn2 mab
    Pkn2 Mab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pkn2+mab/PKN2+Antibody+(11H3)/pmc04733087__mmc1-132-10-19
    Average 90 stars, based on 2 article reviews
    pkn2 mab - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Knock-Out:

    Article Title: Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion
    Article Snippet: .. Frozen section of E10 knockout (-‐/-‐) and wt embryos were stained with anti-‐PKN2 mAb (R&D systems) and an Alexa-‐488 secondary antibody. ..

    Staining:

    Article Title: Knockout of the PKN Family of Rho Effector Kinases Reveals a Non-redundant Role for PKN2 in Developmental Mesoderm Expansion
    Article Snippet: .. Frozen section of E10 knockout (-‐/-‐) and wt embryos were stained with anti-‐PKN2 mAb (R&D systems) and an Alexa-‐488 secondary antibody. ..



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    Image Search Results


    Antibodies used

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Protein kinase N2 regulates AMP kinase signaling and insulin responsiveness of glucose metabolism in skeletal muscle

    doi: 10.1152/ajpendo.00147.2017

    Figure Lengend Snippet: Antibodies used

    Article Snippet: Antibodies used are given in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Target Catalog No. Company PKN2 (cells) 8697 Cell Signaling Technology PAX7 27–583 Prosci DES 15200 Abcam β-Actin A5541 Sigma p-PKN1/2 (Thr 774/816 ) 2611 Cell Signaling Technology p-Akt (Ser 473 ) 9271 Cell Signaling Technology p-Akt (Thr 308 ) 4056 Cell Signaling Technology Akt 9272 Cell Signaling Technology Phospho-GSK-3α/β (Ser 21/9 ) 9331 Cell Signaling Technology GSK-3β 9315 Cell Signaling Technology p-TBC1D4 8619 Cell Signaling Technology TBC1D4 07–741 EMB Millipore p-mTOR (Ser 2448 ) 5536 Cell Signaling Technology mTOR (7C10) 2983 Cell Signaling Technology p-AMPK (Thr 172 ) 2531 Cell Signaling Technology AMPK 2532 Cell Signaling Technology p-ACC (Ser 79 ) 3661 Cell Signaling ACC 3676 Cell Signaling Technology Fyn sc-16 Santa Cruz Biotechnology GAPDH 25778 Santa Cruz Biotechnology p-S6 (Ser 235/236 ) 2211 Cell Signaling Technology S6 2317 Cell Signaling Technology PKN2 (mouse muscle) 2612 Cell Signaling Technology Open in a separate window PKN2, protein kinase N2; DES, desmin; mTOR, mammalian target of rapamycin; AMPK, AMP-activated protein kinase; ACC, acetyl-CoA carboxylase; p, phosphorylated.

    Techniques:

    Protein kinase N2 (PKN2) knockdown decreases insulin responsiveness of glucose metabolism in skeletal muscle. A and B: mRNA levels of PKN2, PAX7, MYOG (myogenin), and DES (desmin) (A) and protein abundance of PKN2, PAX7, and DES (B) in small-interfering (si)RNA-treated primary HSMCs. C: representative bright-field images of siRNA-treated primary human skeletal muscle cells (HSMC). Scale bar, 100 µm. D–F: basal and insulin-stimulated (120 nM) glucose uptake (D), incorporation into glycogen (E), and oxidation (F) in siRNA-treated primary HSMC. Open bars, scrambled (SCR); black bars, small-interfering (si)PKN2. *PKN2 effect, P < 0.05; #insulin main effect, P < 0.05. Results are means ± SE for n = 5 biological replicates.

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Protein kinase N2 regulates AMP kinase signaling and insulin responsiveness of glucose metabolism in skeletal muscle

    doi: 10.1152/ajpendo.00147.2017

    Figure Lengend Snippet: Protein kinase N2 (PKN2) knockdown decreases insulin responsiveness of glucose metabolism in skeletal muscle. A and B: mRNA levels of PKN2, PAX7, MYOG (myogenin), and DES (desmin) (A) and protein abundance of PKN2, PAX7, and DES (B) in small-interfering (si)RNA-treated primary HSMCs. C: representative bright-field images of siRNA-treated primary human skeletal muscle cells (HSMC). Scale bar, 100 µm. D–F: basal and insulin-stimulated (120 nM) glucose uptake (D), incorporation into glycogen (E), and oxidation (F) in siRNA-treated primary HSMC. Open bars, scrambled (SCR); black bars, small-interfering (si)PKN2. *PKN2 effect, P < 0.05; #insulin main effect, P < 0.05. Results are means ± SE for n = 5 biological replicates.

    Article Snippet: Antibodies used are given in . table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Target Catalog No. Company PKN2 (cells) 8697 Cell Signaling Technology PAX7 27–583 Prosci DES 15200 Abcam β-Actin A5541 Sigma p-PKN1/2 (Thr 774/816 ) 2611 Cell Signaling Technology p-Akt (Ser 473 ) 9271 Cell Signaling Technology p-Akt (Thr 308 ) 4056 Cell Signaling Technology Akt 9272 Cell Signaling Technology Phospho-GSK-3α/β (Ser 21/9 ) 9331 Cell Signaling Technology GSK-3β 9315 Cell Signaling Technology p-TBC1D4 8619 Cell Signaling Technology TBC1D4 07–741 EMB Millipore p-mTOR (Ser 2448 ) 5536 Cell Signaling Technology mTOR (7C10) 2983 Cell Signaling Technology p-AMPK (Thr 172 ) 2531 Cell Signaling Technology AMPK 2532 Cell Signaling Technology p-ACC (Ser 79 ) 3661 Cell Signaling ACC 3676 Cell Signaling Technology Fyn sc-16 Santa Cruz Biotechnology GAPDH 25778 Santa Cruz Biotechnology p-S6 (Ser 235/236 ) 2211 Cell Signaling Technology S6 2317 Cell Signaling Technology PKN2 (mouse muscle) 2612 Cell Signaling Technology Open in a separate window PKN2, protein kinase N2; DES, desmin; mTOR, mammalian target of rapamycin; AMPK, AMP-activated protein kinase; ACC, acetyl-CoA carboxylase; p, phosphorylated.

    Techniques: